ارزيابي اثرات ضد قارچ كانديداي نانو ذرات سلنيوم ادغام شده رزين هاي اكريلي: يك مطالعه آزمايشگاهي

Background. Candida albicans in the oral cavity causes denture-induced stomatitis, but current treatments have side effects and contribute to drug resistance. Selenium nanoparticles (SeNPs) show promise as an antimicrobial agent, but their effectiveness against C. albicans is unknown and warrants further research. Methods. Acrylic resins containing different concentrations of selenium nanoparticles (0.2, 2, and 10 g/mL) were formulated and eva‎luated against C. albicans isolates. The minimum inhibitory concentration (MIC) of selenium nanoparticles was determined, and a fungal biofilm was developed on acrylic samples. The quantity of biofilm was assessed using scanning electron microscopy (SEM) and optical density (OD) at 570 nm after staining with crystal violet. Statistical analysis was performed using STATA software, with Kruskal-Wallis and MannWhitney tests to establish significance (P<0.05). Results. The MIC of selenium nanoparticles was 25%. The OD in the group with 10% selenium nanoparticles was 0.477 and 0.547 in the group with 0.2%. Kruskal-Wallis test results showed that at least two groups among those studied had significant differences (P=0.0273). In pairwise comparisons, the differences between all the groups were statistically significant (P=0.049). SEM analysis confirmed the destruction of C. albicans cell walls, leading to reduced colonization, with the 10% group showing the highest efficacy. Conclusion. The study demonstrated that SeNPs are effective against C. albicans colonization when combined with acrylic resin. Specifically, SeNPs exhibited enhanced antifungal properties at a concentration of 10%. These findings confirm that SeNPs are a promising alternative to traditional antifungal agents for treating oral candidiasis and denture-induced stomatitis. Key words: Acrylic resin, Candida, denture, selenium nanoparticles

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نويسنده اول : معصومه رستم زاده